Производитель: J.T.Baker

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Информация для заказа:

1. SILICA REVERSE PHASE PROCESS MEDIA WITH ORDERED POLYMERIC BONDING HIGHLY STABLE OVER AN EXTENDED PH RANGE 

 

Product Number

 

 

Product Name/ Description

 

 

Matrix Properties

pH

Range

Available Pack Sizes

High performance purification of peptides and other small organic molecules based on hydrophobic characteristics and molecular shapes.

7484

C18, ordered poymeric, end-capped

10 μ, 120 Å, spherical silica

2-8.5

100 g (7484-00); 1 kg (74 8 4-01)

7485

C18, ordered poymeric, end-capped

15-30 μ, 120 Å, spherical silica

2-8.5

10 g (7485-02); 1 kg (7485-01)

Economic purification of hydrophilic peptides and proteins.

7025

C18, ordered polymeric, end-capped

40 μ, 60 Å, irregular silica

2-8.5

100 g (7025-00); 1 kg (7025-01); 2 kg 7025-02); 10 kg (7025-10)

Economic purification of hydrophilic organic molecules.

7579

C18, ordered po ymeric, non end-capped

50 μ, 120 Å, spherical silica

2-8.5

1 kg (7579-01); 15 kg (7579-15)

7636

C18, ordered poymeric, end-capped

50 μ, 120 Å, spherical silica

2-8.5

100 g (7636-00); 1 kg (7636-01);

15 kg (7636-15)

High performance purification of strongly hydrophilic peptides and proteins based on hydrophobic characteristics and molecular shapes.

7191

C18, ordered poymeric, end-capped

15 μ, 300 Å, spherical silica

2-8.5

500 g (7191-05); 5 kg (7191-07); Other sizes available

7207

C18, ordered poymeric, end-capped

15-30 μ, 300 Å, spherical silica

2-8.5

500 g (7207-05); 5 kg (7207-07);

10 kg (7207-08); Other sizes

available

Economic purification of hydrophilic peptides and proteins.

7247

C18, ordered poymeric, end-capped

50 μ, 300 Å, spherical silica

2-8.5

100 g (7247-03); 1 kg (7247-01);

Other sizes available

Economic purification of moderately hydrophobic organic molecules.

7901

C8, ordered polymeric, end-capped

50 μ, 120 Å, spherical silica

2-8.5

1 kg (7901-01); 4 kg (7901-04); 5 kg (7901-05);15 kg (7901-15)

High performance purification of moderately hydrophobic proteins and peptides.

7190

C8, ordered polymeric, end-capped

15 μ, 300 Å, spherical silica

2-8.5

10 g (7190-02); 500 g (7190-05)

High performance purification of strongly hydrophobic proteins and peptides.

7179

C4, ordered polymeric, end-capped

15 μ, 300 Å, spherical silica

2-8.5

500 g (7179-05); 5 kg (7179-07);

10 kg (7179-08); Other sizes

available

2. SILICA BASED HYDROPHOBIC INTERACTION (HIC) MEDIA

 

Product Number

Product Name/

Description

 

 

Matrix Properties

pH

Range

Available Pack Sizes

Purification of proteins based on differential salt induced hydrophobicity

7182

Hi-Pro pyl™

15 μ, 300 Å, spherical silica

2-9.0

10 g (7182-02); 500g (7182-05)

7285

Hi-Propyl

40 μ, 275 Å, irregular silica

2-9.0

500 g (7285-05); 1 kg (7285-01);

5 kg (7285-05) Other sizes

available

3. POLYMERIC BASED HYDROPHOBIC INTERACTION (HIC) MEDIA

 

Product Number

Product Name/ Description

Matrix Properties

pH

Range

Available Pack Sizes

Purification of proteins based on differential salt induced hydrophobicity

7588

Poly HI-Propyl

35 μ, 500 Å, spherical polymer

4.5-14.0

50 mL (7688-01); 100 mL (7288-02);

500 mL (7688-03); 5 L (7688-05)

4. SILICA BASED ION EXCHANGERS WITH HYDROPHILIC PEI CHEMISTRY, MIXED MODE, HIGH LINEAR VELOCITIES AND ION EXCHANGE CAPACITY

 

Product Number

 

 

Product Name/ Description

 

 

Matrix Properties

 

 

pH Range

 

 

Available Pack Sizes

Weak cation exchanger with capacity of 0.12 meq/ml.

7157

PEI & carboxylic acid

15 μ, 300 Å, spherical silica

 

 

2-9.0

500 g (7157-05); 5 kg (7157-07);

10 kg (7157-10);

Other sizes available

Weak anion exchanger with capacity of 0.25 meq/ml.

7180

Polyethylene imine (PEI)

15 μ, 300 Å, spherical silica

 

 

2-9.0

10 g (7180-02);

100 g (7180-00); 500 g (7180-05);

Other sizes available

Strong anion exchanger for separation of acidic proteins. Cap. of 0.25 meq/ml.

7183

PEI & quaternary amine

15 μ, 300 Å, spherical silica

 

 

2-9.0

10 g (7183-02);

Other sizes available

Strong cation and weak cation and weak anion exchanger for separation of weakly to highly basic proteins with close isoelectric point. Cap. of 0.2 meq/ml.

7184

Carboxy-Sulfon

15 μ, 300 Å, spherical silica

 

 

2-9.0

10 g (7184-02);

500 g (7184-05);

Other sizes available

7252

Carboxy-Sulfon

40 μ, 275 A, irregular silica

 

 

2-9.0

10 g (7252-02);

100 g (7252-00); 500 g (7252-05); 1 kg (7252-00);

Other sizes available

Strong anion exchanger for separation of acidic proteins with anion exchange capacity of 0.25 meq/ml.

7472

DEAM

15 μ, 300 Å, spherical silica

 

 

2-9.0

10 g (7472-02);

1 kg (7472-00);

Other sizes available

5. POLYMERIC ION EXCHANGERS WITH HYDROPHILIC PEI CHEMISTRY, MIXED MODE, HIGH LINEAR VELOCITIES AND ION EXCHANGE CAPACITY

 

 

Product

Number

Product Name/ Description

 

 

Matrix Properties

 

 

pH

Range

Available Pack Sizes

Poly ABx weak cation and weak anion exchanger for selective purification of proteins and antibodies

7586

Poly ABx (PEI & carboxylic acid)

35 μ, 500 Å, spherical polymer

 

 

2-14.0

50 mL (7586-01); 100 mL (7586­02); 500 mL (7586-03);

5 L (7586-05)

PolyPEI, weak anion exchanger for purification of acidic proteins and peptides, endotoxins, DNA

7585

Poly PEI (Polyethylene imine)

35 μ, 500 Å, spherical polymer

 

 

2-14.0

50 mL (7585-01); 100 mL (7585­02); 500 mL (7585-03);

5 L (7585-05)

PolyCSx, primary strong cation plus weak cation and weak anion exchanger for purification of basic proteins

7587

Poly CSX (PEI & carboxylic acid and sulfonic acid)

35 μ, 500 Å, spherical polymer

 

 

2-14.0

50 mL (7587-01); 100 mL (7587­02); 500 mL (7587-03);

5 L (7587-05)

PolyQuat, strong anion exchanger for purification of acidic proteins, endotoxins and DNA

7603

PolyQuat (PEI & quaternary amine)

35 μ, 500 Å, spherical polymer

 

 

2-14.0

50 mL (7603-01); 100 mL (7603­02); 500 mL (7603-03); 5 L (7603-05)

Информация для выбора необходимого материала для хроматографии
Selection Guide for BAKERBOND™ Process Chromatography Media

Media Selection Tips

Particle Size /Distribution

Particle size and particle distribution influence column efficiency, separation ability and operating velocity. Particle size and distribution also impact operating pressure. Smaller particles result in higher column pressure as compared to larger particles. However, larger average particle sizes with wide distribution (higher content of fines) can also cause higher pressure drops. Average particle size of 30 pm or higher with a particle size distribution of less than 1.5 can be used in low pressure columns (less than 6 bar). Average particle size of 15 pm to 30 pm can be used in medium pressure columns while particles of less than 15 pm require high pressure columns.

Typically, large particles are employed in the beginning of the purification process where large amounts of product need to be purified. Small particles are found in the final purification or polishing step, where smaller amounts of product are being processed. This requires high pressure columns, but of smaller size. Often closely eluting compounds are removed in this step, calling for high efficiencies and therefore small particles. This has the added advantage of yielding narrow bands with highly concentrated product.

Pore Size

Pores should be matched to the molecules to be separated. Interactions between these molecules and the stationary phase take place on the pore surface. While smaller pores offer higher surface areas and therefore capacity, the surface area has to be completely accessible to be effective. This is of particular importance where good mass transfer needs to be maintained at high linear velocities. If particles are not fully porous, larger beads will limit breakthrough capacity at higher linear velocities. Other-wise the dynamic binding capacity will be limited to low flow rates.

Media Shape

Irregular media include fines which have the potential to clog column frits. It thereby increases column pressure and causes inhomogeneous flow (instead of plug flow) within the column bed. This in turn leads to channeling, peak asymmetry as well as to an overall bed instability and further creation of fines. While irregular media tends to be more economical, it typically has a shorter life time compared to spherical media. Spherical media offers lower bed pressure, longer column life and is easier to pack.

Media Base Matrix

Silica matrix offers superior mechanical strength for column operation at high liner velocity and ease in column packing. In addition, it has unique selectivities due to secondary silica interactions. It offers the ability to use organic solvents such as alcohols as well as cleaning solutions for hydrophobic proteins. It can be cleaned using dilute acids, salts and chaotropic reagents such as urea and guanidine hydro-chloride.

Avantor Performance Materials polymeric (non silica) matrix offers high mechanical strength as compared to other soft polymeric resins. As a result It can be operated at a high linear velocity without generating excessive pressure. In addition, it can be operated over a wide pH range of 2 to 14 and can be cleaned or CIP with all common reagents including organic solvents, acids and bases (NAOH).

Таблица соответствия сорбентов для ТФЭ по фирмам производителям (Bakerbond)

JT BAKER
Bakerbond

Phenomenex
strata

Waters
Set-Pak

Varian
Bond Elut

Supelco
Discovery

Agilent
Accubond

IST
Isolute

Macherey-Nagel
Chromabond

обращенно-фазовый

Octadecyl

C18-E

tC18

C18

ENVI-18
LC-18

-

C18(EC)

C18 ec

PolarPlus
Octadecyl Lightload

C18-U

-

-

-

C18

C18

C18
C18 PAH

-

C18-T

C18

-

-

-

-

-

Octyl

C8

C8

C8

LC-8
ENVI-8

C8

C8(EC)

C8

Phenyl

Phenyl (PH)

-

PH

LC-PH

Phenyl

PH(EC)

-

H2O-phobic DVB

SDB-L

-

ENV
LMS

Envi-Chrom P

-

101

HR-P
PS-RP

H2O-philic DVB

strata-X

Oasis HLB
Porapak RDX

NEXUS
PPL
Focus

-

-

ENV+

Easy

нормально-фазовый

Silica Gel

Si-1(Silica)

-

SI

LC-SI

Silica

Silica

SiOH

Florisil

FL-RP

Florisil

Florisil

Florisil

Envi-Florisil

FL

Florisil

Amino

NH2

NH2

NH2

LC-NH2

Amino

NH2

NH2

Cyano

CN

CN

CN-U

LC-CN

Cyano

CN

CN

ионный обмен

Quarternary Amine

SAX

-

SAX

LC-SAX

SAX

SAX

SB

Aromatic Sulfonic Acid

SCX

-

SCX

LC-SCX

SCX

SCX

SA

Carboxylic Acid

WCX

-

CBA

LC-WCX

-

CBA

PCA

Narc-2

Screen-C

-

Cetrify I

-

Evidex

HCX

Drug

Narc-1

Screen-A

-

Cerify II

-

-

HAX

-